October 2022 Progress Update
Experiments in initialization
We’ve been heads-down this month making progress toward our first year goals.
A few highlights:
We welcomed several new team members. With these additions, we now have internal experts across each of our core competence areas.
Yana Blokhina as a Scientist
Alejandro Mendez-Mancilla as a Senior Scientist
Three new computational scientists, by coincidence all joining us in January
We welcomed E. John Wherry to our Scientific Advisory Board!
We ran our first single cell genomics experiments
Single cell genomics sits at the heart of our discovery engine at NewLimit. We’re establishing two in-house chemistries: one focused on ultra high-throughput profiling, and another focused on high-content multi-omic read-outs.
This month, we ran the first of each type of experiment. We look forward to sharing results soon!
Intermediate quality control (QC) testing of cDNA from a single cell genomics profiling experiment. We love watching these curves read-out live.
We also ran the first experiments for our Immunology program and began setting up relevant functional assays
A field of proliferating T cells viewed under a phase contrast microscope. Added an additional set of capabilities to our first perturbation library. Originally constructed in September, we’ve since combined two unique barcoding strategies to enable perturbation experiments that capture a history of cell states, as opposed to just a single snapshot.
We ran some of our first large scale manufacturing experiments to package our perturbation library into viral particles for delivery.
We build with both base pairs and bits at NewLimit, and the two domains are distinct at first blush — lots of liquid in the former, ideally zero liquid in the latter. We believe that a common set of output-focused production principles can be applied across domains, and we’re beginning to see the fruits of that structure play out.
An incubator full of cells serving as microscopic viral packaging factories. 
We’re manufacturing our libraries at bench scale as we ramp up, but we’re excited about an automated system that’s in the works. Just as our LabOps team finished polishing our lab, we began remodeling our office, ensuring a local conservation of entropy.
